The midfielder was initially cited by the match review panel for tackling Adelaide's Patrick Dangerfield into the MCG turf last Sunday, in an incident that resulted in the Crows star being subbed out of the game with concussion.
Depsite being offered a two-match suspension if he lodged an early guilty plea, Trengove contested the penalty at the tribunal on Tuesday night but was unsuccessful.
Melbourne subsequently decided to appeal the tribunal's verdict on three grounds: the decision was so unreasonable that no tribunal could have come to that decision having regard to the evidence; the classification of the level of the offence was manifestly excessive; and the sanction imposed was manifestly excessive.
In a hearing that lasted less than an hour, high-profile lawyer David Galbally QC, who acted on behalf of Trengove, tabled a fresh injury report from the Crows that stated Dangerfield was fit to play this weekend.
He also argued that the board had the ability to overturn or downgrade the penalty if it saw fit.
But the appeals board - made up of Peter O'Callaghan QC, Brian Collis QC and four-time Richmond premiership player Michael Green - deliberated for only seven minutes before finding the tribunal had followed the appropriate process and dismissing the case.
Speaking after the hearing at Etihad Stadium, Trengove said he was "obviously very disappointed with the result.
"Nobody likes to miss any games of footy when they've got the opportunity of playing.
"I suppose because I'm fighting fit and feeling really good it's even more disappointing that I can't get out there with the boys."
Trengove was adamant he would not be changing the way he tackles in future.
"I'm still going to go about my footy the same way and tackle with aggression," he said. "But I suppose if an accident like that happened again then this might happen all over again."
The 19-year-old, who had never previously been suspended at any level of football, said he was delighted with the support he had received from his teammates, many of whom were vocal on Twitter after the initial tribunal hearing.
"It just shows how tight-knit our group is down at the Melbourne footy club," Trengove said.
"They're disappointed as well and they're sticking up for me I suppose."
