Under the guidance of fitness guru Darren Burgess over the past 18 months, the Power have developed a reputation as the fittest and fastest-finishing team in the AFL.
In contrast, North lost a string of close games last season, with some commentators suggesting they did not have the fitness to sustain their hard-running game plan for four quarters.
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The Roos also look set to take on Port without vice-captain Jack Ziebell (shoulder).
Scott said North would delay making a decision on the Ziebell's fitness until as late as Sunday morning, but stressed he would take a safety-first approach at the selection table.
The Roos coach acknowledged Port deserved its reputation as a last-quarter specialist when he spoke with reporters at Arden Street on Thursday, but said he had "the utmost confidence" in North's ability to run out games.
"We've had our hardest pre-season in the five years that I've been here and we pushed the guys to new levels," Scott said.
"Port have shown the ability to have trust and faith in their program and get the job done late in games. Our next step is – we've done the work – it's about actually producing that late in games.
"Hopefully it is tight and tough in the last quarter and we can prevail because I think it would be a great vote of confidence in our conditioning staff."
Scott acknowledged the Roos had yet to hit their best form in 2014 after a dismal round one loss to Essendon and a scrappy win over the Western Bulldogs last Sunday.
But he said the Bulldogs had not allowed North to play an attacking brand of footy and was pleased his team had been able to adapt.
Scott was also confident his team's best was not too far away.
"I think it's something that will click because our preparation has been really good, so it's just a matter of getting a few things right, and once we do I think everything will flow from there pretty quickly," he said.
"I'm confident we'll put a really good performance in this Sunday and we're going to need to against a very good opposition."
Meanwhile, Scott says that if, as expected, the AFL breaks with tradition and schedules a game on Good Friday next year, North Melbourne expects to be part of it.
Bulldogs president Peter Gordon told News Ltd on Thursday that his club also wanted a slice of any Good Friday action.
Gordon acknowledged North had already put in a joint submission with Carlton to play on Good Friday next year, but said the Bulldogs could be part of a second game against another smaller club like St Kilda or Melbourne.
Scott respected the Bulldogs' right to "do the best by their footy club" but said they were "20 years behind North Melbourne".
"We've had really detailed discussions with Carlton in the last couple of years around this game. If and when it happens, we expect it to be a North Melbourne-Carlton game," he said.
"We first came up with the concept as a footy club for Friday night footy and part of that concept was playing a game on Good Friday and raising money for the [Royal] Children's Hospital Appeal.
"So we expect that that's a format that's been really thought out over a long period of time.
"It's good other clubs are putting their hands up, but they might have to wait 20 years like we have."
